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Guide to Protein Purification, 2nd Edition
Chapter 30 Isoelectric Focusing and Two-Dimensional Gel Electrophoresis
edited_book
Author(s):
David B. Friedman
,
Sjouke Hoving
,
Reiner Westermeier
Publication date
(Print):
2009
Publisher:
Elsevier
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Author and book information
Book Chapter
Publication date (Print):
2009
Pages
: 515-540
DOI:
10.1016/S0076-6879(09)63030-5
SO-VID:
719cb6b6-0a93-49c8-b277-50545de0f61e
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Book chapters
pp. i
Series Editors
pp. iv
Copyright Page
pp. liv
Preface to Chapter 1
pp. v
Contents
pp. xix
Contributors to Volume 463
pp. xxv
Preface
pp. xxvii
Volume in Series
pp. 3
Chapter 1 Why Purify Enzymes?
pp. 9
Chapter 2 Strategies and Considerations for Protein Purifications
pp. 21
Chapter 3 Use of Bioinformatics in Planning a Protein Purification
pp. 29
Chapter 4 Preparing a Purification Summary Table
pp. 37
Chapter 5 Setting Up a Laboratory
pp. 43
Chapter 6 Buffers
pp. 57
Chapter 7 Measurement of Enzyme Activity
pp. 73
Chapter 8 Quantitation of Protein
pp. 97
Chapter 9 Concentration of Proteins and Removal of Solutes
pp. 121
Chapter 10 Maintaining Protein Stability
pp. 131
Chapter 11 Selecting an Appropriate Method for Expressing a Recombinant Protein
pp. 149
Chapter 12 Bacterial Systems for Production of Heterologous Proteins
pp. 169
Chapter 13 Expression in the Yeast Pichia pastoris
pp. 191
Chapter 14 Baculovirus–Insect Cell Expression Systems
pp. 223
Chapter 15 Recombinant Protein Production by Transient Gene Transfer into Mammalian Cells
pp. 239
Chapter 16 Tagging for Protein Expression
pp. 259
Chapter 17 Refolding Solubilized Inclusion Body Proteins
pp. 285
Chapter 18 Advances in Preparation of Biological Extracts for Protein Purification
pp. 305
Chapter 19 Isolation of Subcellular Organelles and Structures
pp. 331
Chapter 20 Protein Precipitation Techniques
pp. 343
Chapter 21 Affi-Gel Blue for Nucleic Acid Removal and Early Enrichment of Nucleotide Binding Proteins
pp. 349
Chapter 22 Ion-Exchange Chromatography
pp. 373
Chapter 23 Gel Filtration
pp. 387
Chapter 24 Protein Chromatography on Hydroxyapatite Columns
pp. 405
Chapter 25 Theory and Use of Hydrophobic Interaction Chromatography in Protein Purification Applications
pp. 417
Chapter 26 Affinity Chromatography
pp. 439
Chapter 27 Immobilized-Metal Affinity Chromatography (IMAC)
pp. 475
Chapter 28 Identification, Production, and Use of Polyol-Responsive Monoclonal Antibodies for Immunoaffinity Chromatography
pp. 497
Chapter 29 One-Dimensional Gel Electrophoresis
pp. 515
Chapter 30 Isoelectric Focusing and Two-Dimensional Gel Electrophoresis
pp. 541
Chapter 31 Protein Gel Staining Methods
pp. 565
Chapter 32 Elution of Proteins from Gels
pp. 573
Chapter 33 Performing and Optimizing Western Blots with an Emphasis on Chemiluminescent Detection
pp. 603
Chapter 34 Detergents
pp. 619
Chapter 35 Purification of Membrane Proteins
pp. 631
Chapter 36 Purification of Recombinant G-Protein-Coupled Receptors
pp. 647
Chapter 37 Cell-Free Translation of Integral Membrane Proteins into Unilamelar Liposomes
pp. 677
Chapter 38 Determination of Protein Purity
pp. 691
Chapter 39 Determination of Size, Molecular Weight, and Presence of Subunits
pp. 725
Chapter 40 Identification and Quantification of Protein Posttranslational Modifications
pp. 767
Chapter 41 Parallel Methods for Expression and Purification
pp. 787
Chapter 42 Techniques to Isolate O2-Sensitive Proteins
pp. 809
Chapter 43 Rethinking Your Purification Procedure
pp. 813
Chapter 44 Important but Little Known (or Forgotten) Artifacts in Protein Biochemistry
pp. 821
Author Index
pp. 835
Subject Index
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