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    Review of '<b>A review: CRISPR/Cas12-Mediated Genome Editing in Fungal Cells: Advancements, Mechanisms, and Future Directions in Plant-Fungal Pathology </b>'

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    <b>A review: CRISPR/Cas12-Mediated Genome Editing in Fungal Cells: Advancements, Mechanisms, and Future Directions in Plant-Fungal Pathology </b>Crossref
    The article is discuss the applications of CRISPR/Cas12 system in fungus
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    A review: CRISPR/Cas12-Mediated Genome Editing in Fungal Cells: Advancements, Mechanisms, and Future Directions in Plant-Fungal Pathology

    The CRISPR-associated protein (CRISPR/Cas) system, characterized by clustered regularly interspaced short palindromic repeats, has revolutionized life science research by providing vast possibilities for alteringspecific DNA or RNA sequences in a variety of organisms. The present system integrates fragments of exogenous DNA, known as spacers, into CRISPR cassettes. These cassettes are subsequently transcribed into CRISPR arrays, which are further processed to generate guide RNA (gRNA). The CRISPR arrays are genetic loci that are responsible for encoding Cas proteins. The Cas proteins are responsible for supplying the necessary enzymatic machinery to acquire new spacers that are aimed at invading elements. The development of novel genome engineering tools has been made possible by utilizing various Cas proteins, including but not limited to Cas9, Cas12, Cas13, and Cas14, which possess programmable sequence specificity. The emergence of Cas variants has spurred genetic research and advanced the utilization of the CRISPR/Cas tool to manipulate and edit nucleic acid sequences within a wide range of living organisms. This review aims to furnishoperational modalities ofthe Cas12 protein identified thus far. Furthermore, the advantages and disadvantages ofCas12proteinare examined, along with their recent implementations in the plant fungal world. This review provides the researcher with insights into diverse Cas proteins and their potential applications in advanced genome editing for the next generation. The CRISPR-associated protein (CRISPR/Cas) system, characterized by clustered regularly interspaced short palindromic repeats, has revolutionized life science research by providing vast possibilities for alteringspecific DNA or RNA sequences in a variety of organisms. The present system integrates fragments of exogenous DNA, known as spacers, into CRISPR cassettes. These cassettes are subsequently transcribed into CRISPR arrays, which are further processed to generate guide RNA (gRNA). The CRISPR arrays are genetic loci that are responsible for encoding Cas proteins. The Cas proteins are responsible for supplying the necessary enzymatic machinery to acquire new spacers that are aimed at invading elements. The development of novel genome engineering tools has been made possible by utilizing various Cas proteins, including but not limited to Cas9, Cas12, Cas13, and Cas14, which possess programmable sequence specificity. The emergence of Cas variants has spurred genetic research and advanced the utilization of the CRISPR/Cas tool to manipulate and edit nucleic acid sequences within a wide range of living organisms. This review aims to furnishoperational modalities ofthe Cas12 protein identified thus far. Furthermore, the advantages and disadvantages ofCas12proteinare examined, along with their recent implementations in the plant fungal world. This review provides the researcher with insights into diverse Cas proteins and their potential applications in advanced genome editing for the next generation.
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      Review information

      10.14293/S2199-1006.1.SOR-AG.AHHBQP.v1.RRJZVA
      This work has been published open access under Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com.

      Crops,Agriculture,Molecular biology,Biotechnology,Pests, Diseases & Weeds,Horticulture,Life sciences
      Plant pathogens,Fungal pathogens,CRISPR,CRISPR/Cas12

      Review text

      The article titled "CRISPR/Cas12-Mediated Genome Editing in Fungal Cells: Advancements, Mechanisms, and Future Directions in Plant-Fungal Pathology" explores recent developments in genome editing. It provides an insightful overview of the topic, with a specific focus on plant fungal biology.

      To enhance the readability of the article, I have a few suggestions:

      1. The authors should define abbreviations like tracrRNA to ensure clarity for readers.
      2. Whenever possible, the authors can include web links that provide resources for designing guide RNA, aiding readers in accessing relevant information.
      3. The conclusion section is lengthy and can be merged with the Challenges and Considerations section to streamline the article's structure.

      By implementing these suggestions, the article will become more reader-friendly and enhance its overall readability.

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