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Nucleic Acid Protocols Handbook, The
cDNA Library Screening with the Tetramethylammonium Chloride (TMAC) Technique Using Highly Degenerate Oligonucleotide Probes
edited_book
Author(s):
Bent Honoré
,
Peder Madsen
Publication date
(Print):
March 24 2000
Publisher:
Humana Press
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There is no author summary for this book yet. Authors can add summaries to their books on ScienceOpen to make them more accessible to a non-specialist audience.
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Genome Engineering using CRISPR
Author and book information
Book Chapter
Pages
: 389-395
DOI:
10.1385/1-59259-038-1:389
SO-VID:
6739ef3f-b5e0-457e-b374-6b9e027d2e2d
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Book chapters
pp. 3
Isolation of High-Molecular-Weight DNA from Animal Cells
pp. 9
Isolation of mRNA by Affinity Chromatography
pp. 13
Isolation and Purification of DNA from Plants
pp. 17
Purification of Uncontaminated, Intact Plant RNA
pp. 23
An Improved Method to Isolate Mitochondrial RNA from Green Plant Tissue
pp. 29
Isolating Chromosomal DNA from Bacteria
pp. 33
Bacterial DNA Extraction for Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis
pp. 37
Isolation of Fungal Nucleic Acids
pp. 47
Total RNA Isolation from Bacteria
pp. 53
Simultaneous RNA and DNA Extraction from Biopsy Material, Culture Cells, Plants, and Bacteria
pp. 57
Spectrophotometric Analysis of Nucleic Acids
pp. 63
Restriction Endonuclease Digestion of DNA
pp. 67
Agarose Gel Electrophoresis of Nucleic Acids
pp. 71
Preparation of RNA Dot Blots
pp. 73
Native Polyacrylamide Gel Electrophoresis
pp. 77
Southern Blotting of Agarose Gels by Capillary Transfer
pp. 81
Pulsed-Field Gel Electrophoresis
pp. 105
HPLC of DNA and PCR Products
pp. 117
End-Labeling of DNA Fragments
pp. 123
Nick Translation and Random Hexamer Labeling of DNA
pp. 127
Generation of Labeled Probes by Polymerase Chain Reaction
pp. 135
Nonradioactive Oligonucleotide Probe Labeling
pp. 145
Preparation of Direct, Enzyme-Labeled DNA Probes
pp. 149
Random Prime Labeling of DNA Probes with Fluorescein-Tagged Nucleotides
pp. 153
Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Chemiluminescence
pp. 157
Hybridization of Enzyme-Labeled Probes and Detection by Chemiluminescence
pp. 163
Hybridization and Competition Hybridization of Southern Blots
pp. 169
Autoradiography and Fluorography
pp. 177
Formaldehyde Gel Electrophoresis of Total RNA
pp. 181
RNA Probes for the Analysis of Gene Expression
pp. 195
Primer Extension Analysis of mRNA
pp. 201
S1 Mapping Using Single-Stranded DNA Probes
pp. 207
Measurements of Rate of Transcription in Isolated Nuclei by Nuclear "Run-Off" Assay
pp. 213
One-Tube RT-PCR with Sequence-Specific RT Primers
pp. 219
Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA
pp. 229
Quantitative Analysis of RNA Species by Polymerase Chain Reaction and Solid-Phase Minisequencing
pp. 239
Nonradioactive Northern Blotting of RNA
pp. 249
Analysis of RNA by Northern Blotting Using Riboprobes
pp. 261
Production of Double-Stranded cDNA for Gene Library Synthesis
pp. 267
Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs
pp. 289
cDNA Library Construction Using Streptavidin-Paramagnetic Beads and PCR
pp. 295
Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products
pp. 305
Subtraction Hybridization cDNA Libraries
pp. 319
Cloning Polymerase Chain Reaction Products Utilizing the T/A Overhang and a Kit
pp. 327
Extraction and Purification of Plasmid DNA
pp. 333
Biotinylated Probes in Colony Hybridization
pp. 339
Cloning Long Polymerase Chain Reaction Products
pp. 347
Cloning DNA Fragments in M13 Vectors
pp. 355
cDNA Library Construction for the Lambda ZAP®-Based Vectors
pp. 367
Expression and Preparation of Fusion Proteins from Recombinant λgt11 Phages
pp. 373
Antibody Screening of Bacteriophage λgt11 DNA Expression Libraries
pp. 381
Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides
pp. 389
cDNA Library Screening with the Tetramethylammonium Chloride (TMAC) Technique Using Highly Degenerate Oligonucleotide Probes
pp. 397
Screening Recombinant Libraries by Polymerase Chain Reaction
pp. 405
Construction and Screening of Cosmid Libraries
pp. 415
Generation of Large Insert YAC Libraries
pp. 425
YAC Library Storage and Transport
pp. 431
YAC Library Screening: Preparation of Hybridization Filters and Polymerase Chain Reaction Pools
pp. 437
YAC Library Screening: Hybridization and PCR-Based Screening Protocols
pp. 449
Phage-Display Libraries of Murine and Human Antibody Fab Fragments
pp. 481
Preparation and Analysis of DNA Sequencing Gels
pp. 489
DNA Sequencing of Plasmids
pp. 493
Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors
pp. 499
Direct cDNA Sequencing Using Sequential Linear/Asymmetric Polymerase Chain Reaction
pp. 505
Purification and Enzymatic Sequencing of Polymerase Chain Reaction Products
pp. 515
Direct Polymerase Chain Reaction Sequencing with Denaturants
pp. 523
Direct DNA Sequencing of Polymerase Chain Reaction Products Using Magnetic Beads
pp. 533
Polymerase Chain Reaction Cycle Sequencing with Degenerate Primers
pp. 541
Direct Automated Cycle Sequencing of Polymerase Chain Reaction Products
pp. 547
Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products
pp. 553
DNA Sequencing by the Chemical Method
pp. 557
One-Step One-Lane Chemical Sequencing of DNA
pp. 569
Polymerase Chain Reaction: Basic Principles and Routine Practice
pp. 581
Primer Selection and Design for Polymerase Chain Reaction
pp. 589
One-Step Optimization Using Touchdown and Stepdown Polymerase Chain Reaction
pp. 595
Cloning Gene Family Members Using Polymerase Chain Reaction with Degenerate Oligonucleotide Primers
pp. 609
Construction of Synthetic Genes by Polymerase Chain Reaction
pp. 613
Rapid Amplification of cDNA Ends
pp. 619
Multiplex Polymerase Chain Reaction
pp. 625
Inverse Polymerase Chain Reaction
pp. 633
Long-Range Polymerase Chain Reaction
pp. 645
Nonradioactive Differential Display of Messenger RNA
pp. 653
Gene Isolation by Exon Trapping
pp. 667
DNA Rescue by the Vectorette Method
pp. 675
Random Amplified Polymorphic DNA (RAPDs)
pp. 679
Restriction Fragment Length Polymorphism
pp. 685
Detection of Mutations in DNA and RNA by Chemical Cleavage
pp. 695
Mutation Screening Using PCR-SSCP: Silver Staining and Isotopic Protocols
pp. 705
Detecting Point Mutations by Denaturing-Gradient Gel Electrophoresis
pp. 717
Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
pp. 723
The Amplification Refractory Mutation System
pp. 729
DNase I Footprinting
pp. 737
Identification of Protein-DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference
pp. 745
The Gel Shift Assay for the Analysis of DNA-Protein Interactions
pp. 757
Yeast Two-Hybrid Library Screening
pp. 773
The Southwestern Assay
pp. 783
Nonradioactive Methods for the Detection of RNA-Protein Interaction
pp. 793
Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay
pp. 807
Generating Nested Deletions with Exonuclease III
pp. 815
Primer-Directed Site-Specific Mutagenesis
pp. 827
Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template
pp. 835
Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates
pp. 845
Site-Directed Mutagenesis with LA-PCR™ Technology
pp. 857
Recombination and Mutagenesis by Overlap Extension PCR
pp. 865
Site-Directed Mutagenesis and Gene Fusion by Megaprimer PCR
pp. 875
Transcription In Vitro Using Bacteriophage RNA Polymerases
pp. 885
In Vitro Translation of mRNA in a Rabbit Reticulocyte Lysate Cell-Free System
pp. 891
In Vitro Translation of mRNA in a Wheat Germ Extract Cell-Free System
pp. 895
The Xenopus Egg Extract Translation System
pp. 907
Manipulation of Baculovirus Vectors
pp. 921
Procedures for the Analysis and Purification of His-Tagged Proteins
pp. 935
Detection and Immobilization of Proteins Containing the 6xHis Tag
pp. 947
Expression and Purification of Recombinant Proteins Using the pET System
pp. 981
Preparation of Tissue Sections and Slides for mRNA Hybridization
pp. 985
Use of Digoxigenin-Labeled Probes on Tissue Sections
pp. 991
Gene Mapping by FISH
pp. 1011
Oligonucleotide PRINS DNA Synthesis
pp. 1017
Chromosome-Specific PRINS
pp. 1023
In Situ PCR Amplification of Intracellular mRNA
pp. 1031
An Introduction to Bioinformatics
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