Chalcone Synthase: Method Optimization of Extraction and Activity Assay in Strawberry Fruit

Method development and optimization play a central role in analytical chemistry and more specifically in food biochemistry. When it comes to research, it is common that analytical methods need to be modified to specific experimental biological tissues. While there are several published works on the activity of the enzyme chalcone synthase (CHS) in plant materials, such as sweet basil using ultra- high-performance liquid chromatography, there is a lack of information regarding extraction and activity of CHS in strawberries. Therefore, the main objective of this work was to optimize existing published methods for extraction and activity of CHS in strawberries, using spectrophotometric analysis. It was done through a literature search, a method dissection was performed, followed by theoretical optimization of the protocol, and finally an experimental optimization