Gold standard is a very common but most powerful terminology amongst diagnostic and analytical laboratory scientists. The genesis dates back to 19th century monetary system where the standard economic unit of account is based on a fixed quantity of gold and has nothing to do with science. Nevertheless, this term had taken an indispensable place in the testing arena ever since its appearance in reputed scientific journals. Application of this term in analytical science from both proponents and opponents perspective has been discussed in detail. Its applicability with regards to bacteriological culture and pulsed field gel electrophoresis for typing bacteria and clinical diagnosis as examples are presented. In conclusion, diagnostic and analytical test methods should not be described or presented in a misleading manner as it is likely to create an erroneous impression on the use of such terminology. We need to revisit gold standard terminology for its appropriation.