Abattoir Effluent could pose a major environmental and health problem to aquatic systems. The long and short term safety and health effect on humans and livestock depend upon the physical and chemical characteristics of the pollutant and exposure to such polluted water system. The Benue River is non-tidal freshwater ecological system located in Makurdi local government area of Benue State, Nigeria. The study is aimed at presenting results of the physical and chemical parameters for the period of April to July 2019. Parameters such as temperature, turbidity, suspended solids, pH, conductivity, dissolved solid, dissolved oxygen, BOD and COD were obtained. Samples were taken at different points in the river within the distance of 200 meters downstream, midstream, upstream and the point source. The concentrations of the parameters were obtained using standard procedures. The results indicate that the water quality parameters like suspended solid (45.2 mg/L), dissolved oxygen (5.2 mg/L), turbidity (24.1 NTU), fell above the standard stipulated by the World Health Organization (WHO), Nigerian Standard for Drinking Water Quality (NSDWQ) and Standard Organization of Nigeria (SON). Statistical analysis was also used to develop a regression equation, the best model gave a correlation coefficient of 0.887 as SS = 133.304 - 0.583SS – 0.837TS – 2.308Temperature. By application of these standard approaches, TDS and EC show strongly positive correlation because the ions from the dissolve solid in water influenced the ability of the water to conduct an elementary current. These two parameters significantly vary among nine sites which are further confirmed by using linear regression. Hence, the water is unsafe for drinking and domestic use. It is recommended that the water should be treated before it can be used for irrigation purpose.