Image correlation spectroscopy approaches for the quantification of molecular and organellar dynamics in situ

Image correlation spectroscopy (ICS) has been around for over 25 years and is applied to fluorescence microscopy data to quantify molecular concentration, dynamics, (diffusion, flow), chemical reactions and co-localisation. Over that time period there has been an emergence of ICS derived analysis approaches taking advantage of unique spatial and temporal capabilities of different microscopy modalities (Confocal, TIRF, Lightsheet, etc), providing a unique set of measurable parameters about biological systems.

In this talk, I will introduce you to ICS derived approaches that rely on Spatio-Temporal ICS (STICS) analysis resolving molecular and organellar flow in 2D and 3D. Furthermore, we will explore the benefits of its Fourier space counterpart, k-space ICS (kICS) in investigating dynamics of proteins in confined environments.

In the last part of this talk, I will demonstrate how these two approaches can be used to quantify the ciliary beating frequency (CBF) and ciliary spatial coordination length in the context of diagnosis of respiratory disease