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      Quantifying T Cell Activation: Single-molecule binding kinetics of signaling proteins using Protein-PAINT

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      conference-abstract
      Author(s): 1 , , 1 , 1 , 1
      Publication date (Electronic):
      Conference name: 13th Asia Pacific Microscopy Congress 2025 (APMC13)
      Conference date: 2-7 Febuary 2025
      Keywords: Protein-PAINT, Binding Kinetics, Single molecule localisation microscopy, Receptor signalling
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            Abstract

            Content

            Author and article information

            Conference
            Publication date (Electronic): 21 January 2025
            Electronic Location Identifier: e023
            Affiliations
            [1 ]Department of Molecular Medicine, School of Biomedical Sciences, University of New South Wales, Sydney, NSW, Australia
            Author notes
            *Corresponding author: shirin.ansari@ 123456unsw.edu.au
            Author information
            https://orcid.org/0009-0000-1283-4430
            Article
            DOI: 10.14293/APMC13-2025-0023
            SO-VID: 18159c65-0fbf-45ac-86b2-2c6f024d77a2
            Copyright © 2025 The Authors.
            License:

            Published under Creative Commons Attribution 4.0 International ( CC BY 4.0). Users are allowed to share (copy and redistribute the material in any medium or format) and adapt (remix, transform, and build upon the material for any purpose, even commercially), as long as the authors and the publisher are explicitly identified and properly acknowledged as the original source.

            Conference name: 13th Asia Pacific Microscopy Congress 2025
            Conference acronym: APMC13
            Conference number: 13
            Conference location: Brisbane, Australia
            Conference date: 2-7 Febuary 2025
            History
            Categories
            Subject: BLS05 - Super-Resolution & Single Molecule Optical Microscopy

            Keywords: Protein-PAINT,Receptor signalling,Single molecule localisation microscopy,Binding Kinetics

            References

            1. J. Goyette, D. J. Nieves, Y. Ma, K. Gaus, How does T cell receptor clustering impact on signal transduction? J Cell Sci 132 (2019).

            2. M. V. Farrell et al., Protein-PAINT: Superresolution microscopy with signaling proteins. Sci Signal 15, eabg9782 (2022).

            3. S. Coelho et al., Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells. Sci Adv 6, eaay8271 (2020).

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