Evaluation of human embryo development in in vitro fertilization- and intracytoplasmic sperm injection-fertilized oocytes: A time-lapse study

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Abstract

Objective

We investigated whether the insemination method ( in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development.

Methods

A total of 1,830 normal fertilized embryos were obtained from 272 IVF and ICSI cycles that underwent ovum retrieval culture using a time-lapse system (Embryoscope) from June 2013 to March 2015. All embryos were investigated by a detailed time-lapse analysis that measured the developmental events in the hours after IVF or ICSI insemination.

Results

No significant differences were observed between the two groups regarding clinical outcomes ( p>0.05). ICSI-derived embryos showed significantly faster morphokinetics than those derived from conventional IVF, from the time to pronuclear fading to the time to 6 cells ( p<0.05). However, no significant differences were found from the time to 7 cells to the time to expanded blastocyst ( p>0.05). There were no differences in abnormal cleavage events between the two groups ( p>0.05); they showed the same rates of direct cleavage from 1 to 3 cells, 2 multinucleated cells, 2 uneven cells, and reverse cleavage.

Conclusion

The morphokinetics of embryo development was found to vary between IVF- and ICSI-fertilized oocytes, at least until the 6-cell stage. However, these differences did not affect the clinical outcomes of the embryo. Additionally, no significant differences in abnormal cleavage events were found according to the fertilization method.

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Most cited references 25

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Limited implantation success of direct-cleaved human zygotes: a time-lapse study.

Irene Rubio, Reidun Kuhlmann, Inge Agerholm … (2012)

To evaluate embryos with direct cleavage (≤5 hours) from two to three cells (DC2-3) and correlate this morphokinetic parameter to implantation and ongoing pregnancy. Clinical multicenter retrospective study. Private in vitro fertilization (IVF) centers. From three clinics, a total of 979 treatments including 5,225 embryos using autologous or donated oocytes, of which 1,659 embryos were transferred. None. Clinical pregnancy confirmed by ultrasound in week 7. Of the total embryo cohort, 715 (13.7%) underwent direct cleavage from two to three cells, 1,659 embryos were transferred to recipients, and 109 of the transferred embryos cleaved directly from two to three cells (6.6%). Only one DC2-3 embryo was known to result in a clinical pregnancy (1%) and 80 (73.4%) DC2-3 embryos did not implant. Of the 1,550 embryos transferred not showing DC2-3, 203 embryos were from treatments with 100% implantation (13.1%), and 804 (51.8%) embryos did not implant. The known implantation rate of DC2-3 embryos was statistically significantly lower than for embryos with a normal cleavage pattern (1.2% vs. 20.2%, respectively). Embryos with DC2-3 had a statistically significantly lower implantation rate than embryos with a normal cleavage pattern, suggesting that rejection of these embryos for transfer could improve the implantation rate. Copyright © 2012 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

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Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation.

T Hardarson, C. Hanson, A Sjögren … (2001)

Uneven blastomere cleavage in human embryos of 'good morphology', i.e. those normally used for transfer, is a phenomenon which has been poorly investigated. The main objective in this study was to probe deeper into the aetiology behind previous findings that embryos with uneven cell cleavage have a lower developmental capacity in comparison with evenly cleaved embryos. Our hypothesis was that uneven cleavage may result in embryos with a higher degree of aneuploidy and/or multinuclear rate, which in turn might help to explain their low implantation rate. In the first part of the study, 378 embryo transfers performed over a 3-year period were analysed retrospectively, where all the transferred embryos in each cycle were of identical morphology score and cleavage stage. In the second part of the study, multicolour fluorescence in-situ hybridization (FISH) analyses on good quality embryos, representing the uneven (n = 11) and even (n = 13) study groups were performed. When comparing day 2 transfers between 4-cell embryos, it was found that unevenly cleaved embryos had significantly lower implantation (23.9 and 36.4%) and pregnancy rates (37.6 and 52.9%) compared with evenly cleaved embryos. A significantly higher degree of aneuploidy (29.4 and 8.5%) and multinuclear rate (21.1 and 2.1%) in blastomeres from uneven embryos was also found. It is concluded that uneven blastomere cleavage has a negative effect on both pregnancy and implantation rates in human IVF, and that this can partly be explained by a higher degree of aneuploidy/multinuclear rate. In the light of the results obtained, a new approach in the current embryo scoring system, placing more emphasis on blastomere size, is recommended.

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Kinetic markers of human embryo quality using time-lapse recordings of IVF/ICSI-fertilized oocytes.

JG Lemmen, I Agerholm, S Ziebe (2008)

A time-lapse system was used to study the timing and coordination of events during early development from zygote to cleavage stage embryo. The aim was to identify markers linked to good-quality embryos and implantation. A total of 102 fertilized oocytes were followed for 20-24 h. Events such as appearance and disappearance of (pro)nuclei and timing and synchronization of cell cleavage were logged as time points after fertilization. Averages for these events and their synchrony were calculated and linked with fertilization method, embryo quality and implantation success. Fertilized oocytes that developed into > or =4-cell embryos had an earlier pronuclei disappearance and first cleavage than those that developed to 3- or 2-cell embryos. Intracytoplasmic sperm injection-fertilized 4-cell embryos spent a significantly shorter period as 2-cell compared with IVF-fertilized embryos (P = 0.0090). Development in the time-lapse system was similar to their siblings cultured in normal incubators, suggesting that the data from the time-lapse system can be extrapolated to the clinic's laboratory setting. Early disappearance of pronuclei and onset of first cleavage after fertilization was correlated with a higher number of blastomeres on day 2 after oocyte retrieval. In addition, synchrony in appearance of nuclei after the first cleavage was significantly associated with pregnancy success (P < 0.05).

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Author and article information

Journal

Journal ID (nlm-ta): Clin Exp Reprod Med

Journal ID (iso-abbrev): Clin Exp Reprod Med

Journal ID (publisher-id): CERM

Title: Clinical and Experimental Reproductive Medicine

Publisher: The Korean Society for Reproductive Medicine

ISSN (Print): 2233-8233

ISSN (Electronic): 2233-8241

Publication date (Print): June 2017

Publication date (Electronic): 30 June 2017

Volume: 44

Issue: 2

Pages: 90-95

Affiliations

[1 ]Maria Fertility Hospital, Seoul, Korea.

[2 ]Fertility Research Center, Maria Medical Foundation, Seoul, Korea.

Author notes

Corresponding author: Hyung Jun Kim. Maria Fertility Hospital, 20 Cheonho-daero, Dongdaemun-gu, Seoul 02586, Korea. Tel: +82-2-2250-5522, Fax: +82-2-2250-5549, blue@ 123456mariababy.com

Article

DOI: 10.5653/cerm.2017.44.2.90

PMC ID: 5545225

PubMed ID: 28795048

SO-VID: fed96986-fc38-48a0-8b48-185374bc4dcf

License:

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

History

Date received : 17 March 2017

Date revision received : 08 April 2017

Date accepted : 17 May 2017

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Evaluation of human embryo development in in vitro fertilization- and intracytoplasmic sperm injection-fertilized oocytes: A time-lapse study

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Abstract

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Most cited references 25

Limited implantation success of direct-cleaved human zygotes: a time-lapse study.

Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation.

Kinetic markers of human embryo quality using time-lapse recordings of IVF/ICSI-fertilized oocytes.

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