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      Poster 299: Culturing Osteochondral Explants Under Rotatory Shaking or After Removing Bone Marrow Elements Increase Explant Cellular Viability

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          Abstract

          Objectives:

          Reduced viability in the deepest zones of osteochondral allografts (OCAs) can weaken the subchondral interface, potentially causing graft delamination and failure. This reduction may result from nutritional imbalances due to uneven media distribution or interference from bone marrow elements. This study aims to investigate whether culturing OCAs using a rotatory shaker or removing the bone marrow elements increase graft cellular viability.

          Methods:

          Bovine osteochondral (OC) explants were stored for 28 days at 4°C under three different conditions (n = 6 explants/group): static (control group), rotatory shaker at 150 rpm (shaker group), and static after removing bone marrow elements using a waterpik device (waterpik group). Chondrocyte viability was assessed using live/dead staining across the entire tissue and in each zone (superficial, middle, deep zone). Subchondral bone viability was assessed using tunel staining by detecting apoptotic cells.

          Results:

          The use of a rotatory shaker (p = 0.010) or waterpik (p = 0.005) significantly increased chondrocyte viability compared to the static control. When analyzing by zone, both the shaker and the waterpik groups displayed higher cellular viability in the middle (p < 0.001) and deep zone (p = 0.018, shaker; p = 0.025, waterpik) when contrasted with the control group. Furthermore, shaker and waterpik groups demonstrated reduced levels of subchondral bone apoptotic cells compared to control (p = 0.018 and p = 0.007, respectively). Notably, no significant differences were observed between the shaker and waterpik groups in any of the analyses.

          Conclusions:

          These findings indicate that both rotatory shaking and removing bone marrow elements during osteochondral explants storage lead to higher cellular viability at the middle and deep zones of the graft compared to the static storage condition. Enhancing nutrition delivery to the graft could improve its quality, potentially improving outcomes of OCA transplantation.

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          Author and article information

          Journal
          Orthop J Sports Med
          Orthop J Sports Med
          OJS
          spojs
          Orthopaedic Journal of Sports Medicine
          SAGE Publications (Sage CA: Los Angeles, CA )
          2325-9671
          9 October 2024
          July 2024
          : 12
          : 7 suppl2 , AOSSM 2024 Annual Meeting
          : 2325967124S00266
          Affiliations
          [1-2325967124S00266]Orthopaedic Department, Brigham Women’s Hospital
          Article
          10.1177_2325967124S00266
          10.1177/2325967124S00266
          11465325
          fecd0bdf-f961-4d46-8964-5b97fb577515
          © The Author(s) 2024

          This open-access article is published and distributed under the Creative Commons Attribution - NonCommercial - No Derivatives License ( https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits the noncommercial use, distribution, and reproduction of the article in any medium, provided the original author and source are credited. You may not alter, transform, or build upon this article without the permission of the Author(s). For article reuse guidelines, please visit SAGE’s website at http://www.sagepub.com/journalsPermissions.nav.

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