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      Elektronenmikroskopische Untersuchungen zur geotropischen Reaktion : I I. Die polare Organisation des normal wachsenden Rhizoids vonChara foetida

      Protoplasma
      Springer Science and Business Media LLC

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          A STUDY OF FIXATION FOR ELECTRON MICROSCOPY

          G E Palade (1952)
          Osmium tetroxide fixation of tissue blocks, as usually effected, is preceded by an acidification of the tissue. This acidification is probably responsible for morphological alterations which are notably disturbing in electron microscopy. The acidification and the resulting morphological alterations cannot be prevented by homogenizing the tissue directly in OsO4 solutions or by adding enzyme inhibitors (fluoride, iodoscetamide) to the fixative. Fixation experiments with buffered OsO4 solutions have shown that the appearance of the fixed cells is conditioned by the pH of the fixative. The quality of fixation can be materially improved by buffering the OsO4 solutions at pH 7.3-7.5, The acetate-veronal buffer appeared to be the most favorable of the buffers tested, Because of these findings, 1 per cent OsO4 buffered at pH 7.3-7.5 with acetate-veronal buffer is recommended as an appropriate fixative for electron microscopy.
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            Permanganate—A New Fixative for Electron Microscopy

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              Golgi Apparatus and Plant Secretion

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                Author and article information

                Journal
                Protoplasma
                Protoplasma
                Springer Science and Business Media LLC
                0033-183X
                1615-6102
                September 1967
                September 1967
                : 64
                : 3
                : 225-253
                Article
                10.1007/BF01253704
                e64a234d-d848-4402-92c6-b8869bb06163
                © 1967

                http://www.springer.com/tdm

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