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      CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex.

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          Abstract

          The Mi-2/NuRD (NUcleosome Remodeling and histone Deacetylase) chromatin remodeling complex is a large heterogeneous multiprotein complex associated with transcriptional repression. Here we apply a SILAC based quantitative proteomics approach to show that all known Mi-2/NuRD complex subunits co-purify with Cyclin Dependent Kinase 2 Associated Protein1 (CDK2AP1), also known as Deleted in Oral Cancer 1 (DOC-1). DOC-1 displays in vitro binding affinity for methylated DNA as part of the meCpG binding MBD2/NuRD complex. In luciferase reporter assays, DOC-1 is a potent repressor of transcription. Finally, immunofluorescence experiments reveal co-localization between MBD2 and DOC-1 in mouse NIH-3T3 nuclei. Collectively, these results indicate that DOC-1 is a bona fide subunit of the Mi-2/NuRD chromatin remodeling complex.

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          Author and article information

          Journal
          Mol Biosyst
          Molecular bioSystems
          Royal Society of Chemistry (RSC)
          1742-2051
          1742-2051
          Sep 2010
          : 6
          : 9
          Affiliations
          [1 ] Department of Physiological Chemistry and Cancer Genomics Centre, University Medical Center Utrecht, Utrecht, The Netherlands.
          Article
          10.1039/c004108d
          20523938
          e5047398-9399-45d2-a805-742349998968
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