30
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Gastrointestinal digestion of dietary advanced glycation endproducts using an in vitro model of the gastrointestinal tract (TIM-1)

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          In a sophisticated gastrointestinal model, dietary advanced glycation endproducts (dAGEs) in food products remain bound to proteins after digestion and concentrations increase.

          Abstract

          Protein- and sugar-rich food products processed at high temperatures contain large amounts of dietary advanced glycation endproducts (dAGEs). Our earlier studies have shown that specifically protein-bound dAGEs induce a pro-inflammatory reaction in human macrophage-like cells. To what extent these protein-bound dAGEs survive the human gastrointestinal (GI) tract is still unclear. In this study we analysed gastric and small intestinal digestion of dAGEs using the validated, standardised TNO in vitro gastroIntestinal digestion model (TIM-1), a dynamic in vitro model which mimics the upper human GI tract. This model takes multiple parameters into account, such as: dynamic pH curves, peristaltic mixing, addition of bile and pancreatic digestive enzymes, and passive absorption. Samples of different digested food products were collected at different time points after (i) only gastric digestion and (ii) after both gastric plus small intestinal digestion. Samples were analysed for dAGEs using UPLC-MS/MS for the lysine derived N ε-carboxymethyllysine (CML) and N ε-carboxyethyllysine (CEL), and the arginine derived methylglyoxal-derived hydroimidazolone-1 (MG-H1), and glyoxal-derived hydroimidazolone-1 (G-H1). All AGEs were quantified in their protein-bound and free form. The results of this in vitro study show that protein-bound dAGEs survive gastrointestinal digestion and are additionally formed during small intestinal digestion. In ginger biscuits, the presence MG-H1 in the GI tract increased with more than 400%. This also indicates that dAGEs enter the human GI tract with potential pro-inflammatory characteristics.

          Related collections

          Most cited references1

          • Record: found
          • Abstract: not found
          • Book: not found

          Biochemistry

          Berg (2002)
            Bookmark

            Author and article information

            Contributors
            (View ORCID Profile)
            Journal
            FFOUAI
            Food & Function
            Food Funct.
            Royal Society of Chemistry (RSC)
            2042-6496
            2042-650X
            July 22 2020
            2020
            : 11
            : 7
            : 6297-6307
            Affiliations
            [1 ]Department of Pharmacology and Toxicology
            [2 ]Maastricht University
            [3 ]Maastricht
            [4 ]The Netherlands
            [5 ]Centre for Healthy Eating & Food Innovation (HEFI)
            [6 ]Maastricht University - campus Venlo
            [7 ]Venlo
            [8 ]Wageningen Food Safety Research
            [9 ]Wageningen University and Research
            [10 ]Wageningen
            [11 ]Campus Venlo
            [12 ]Office for Risk Assessment and Research
            Article
            10.1039/D0FO00450B
            32602872
            c0ad37d1-36a3-4e11-a66d-a796b328a5e5
            © 2020

            http://creativecommons.org/licenses/by-nc/3.0/

            History

            Comments

            Comment on this article

            scite_
            0
            0
            0
            0
            Smart Citations
            0
            0
            0
            0
            Citing PublicationsSupportingMentioningContrasting
            View Citations

            See how this article has been cited at scite.ai

            scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

            Similar content276

            Cited by18

            Most referenced authors1