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      Recent Advances of CRISPR-Cas9 Genome Editing Technologies for Biological and Biomedical Investigations.

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          Abstract

          The Type II CRISPR-Cas9 system is a simple, efficient, and versatile tool for targeted genome editing in a wide range of organisms and cell types. It continues to gain more scientific interest and has established itself as an extremely powerful technology within our synthetic biology toolkit. It works upon a targeted site and generates a double strand breaks that become repaired by either the NHEJ or HDR pathway, modifying or permanently replacing the genomic target sequences of interest. These can include viral targets, single-mutation genetic diseases, and multiple-site corrections for wide scale disease states, offering the potential to manage and cure some of mankind's most persistent biomedical menaces. Here, we present the developing progress and future potential of CRISPR-Cas9 in biological and biomedical investigations, towards numerous therapeutic, biomedical, and biotechnological applications, as well as some of the challenges within. This article is protected by copyright. All rights reserved.

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          Author and article information

          Journal
          J. Cell. Biochem.
          Journal of cellular biochemistry
          Wiley-Blackwell
          1097-4644
          0730-2312
          May 24 2017
          Affiliations
          [1 ] Synthetic Biology Laboratory, Department of Microbiology, School of Biological Sciences and Biotechnology, Institute of Advanced Research, Koba Institutional Area, Gandhinagar - 382007, India.
          [2 ] Department of R&D, Cementic S.A.S., Paris 75000, France.
          [3 ] Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, Dschang, Cameroon.
          Article
          10.1002/jcb.26165
          28544016
          ba3cc2e5-b2a6-436f-ac68-a91db705afc8
          History

          CRISPR-Cas9,DSB,HDR,Indels,NHEJ,Therapeutic,sgRNA
          CRISPR-Cas9, DSB, HDR, Indels, NHEJ, Therapeutic, sgRNA

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