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      Transparent adult zebrafish as a tool for in vivo transplantation analysis.

      Cell Stem Cell
      Albinism, genetics, Animals, Flow Cytometry, Light, Models, Animal, Neoplasm Transplantation, Pigmentation, Stem Cell Transplantation, Stem Cells, ultrastructure, Zebrafish

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          Abstract

          The zebrafish is a useful model for understanding normal and cancer stem cells, but analysis has been limited to embryogenesis due to the opacity of the adult fish. To address this, we have created a transparent adult zebrafish in which we transplanted either hematopoietic stem/progenitor cells or tumor cells. In a hematopoiesis radiation recovery assay, transplantation of GFP-labeled marrow cells allowed for striking in vivo visual assessment of engraftment from 2 hr-5 weeks posttransplant. Using FACS analysis, both transparent and wild-type fish had equal engraftment, but this could only be visualized in the transparent recipient. In a tumor engraftment model, transplantation of RAS-melanoma cells allowed for visualization of tumor engraftment, proliferation, and distant metastases in as little as 5 days, which is not seen in wild-type recipients until 3 to 4 weeks. This transparent adult zebrafish serves as the ideal combination of both sensitivity and resolution for in vivo stem cell analyses.

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          Author and article information

          Journal
          18371439
          2292119
          10.1016/j.stem.2007.11.002

          Chemistry
          Albinism,genetics,Animals,Flow Cytometry,Light,Models, Animal,Neoplasm Transplantation,Pigmentation,Stem Cell Transplantation,Stem Cells,ultrastructure,Zebrafish

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