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      The Arabidopsis thaliana lectin receptor kinase LecRK‐I.9 is required for full resistance to Pseudomonas syringae and affects jasmonate signalling

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          Summary

          On microbial attack, plants can detect invaders and activate plant innate immunity. For the detection of pathogen molecules or cell wall damage, plants employ receptors that trigger the activation of defence responses. Cell surface proteins that belong to large families of lectin receptor kinases are candidates to function as immune receptors. Here, the function of LecRK‐I.9 (At5g60300), a legume‐type lectin receptor kinase involved in cell wall–plasma membrane contacts and in extracellular ATP (eATP) perception, was studied through biochemical, gene expression and reverse genetics approaches. In Arabidopsis thaliana, LecRK‐I.9 expression is rapidly, highly and locally induced on inoculation with avirulent strains of Pseudomonas syringae pv. tomato ( Pst). Two allelic lecrk‐I.9 knock‐out mutants showed decreased resistance to Pst. Conversely, over‐expression of LecRK‐I.9 led to increased resistance to Pst. The analysis of defence gene expression suggests an alteration of both the salicylic acid (SA) and jasmonic acid (JA) signalling pathways. In particular, LecRK‐I.9 expression during plant–pathogen interaction was dependent on COI1 (CORONATINE INSENSITIVE 1) and JAR1 (JASMONATE RESISTANT 1) components, and JA‐responsive transcription factors (TFs) showed altered levels of expression in plants over‐expressing LecRK‐I.9. A similar misregulation of these TFs was obtained by JA treatment. This study identified LecRK‐I.9 as necessary for full resistance to Pst and demonstrated its involvement in the control of defence against pathogens through a regulation of JA signalling components. The role of LecRK‐I.9 is discussed with regard to the potential molecular mechanisms linking JA signalling to cell wall damage and/or eATP perception.

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          Author and article information

          Contributors
          canut@lrsv.ups-tlse.fr
          Journal
          Mol Plant Pathol
          Mol. Plant Pathol
          10.1111/(ISSN)1364-3703
          MPP
          Molecular Plant Pathology
          John Wiley and Sons Inc. (Hoboken )
          1464-6722
          1364-3703
          15 September 2016
          September 2017
          : 18
          : 7 ( doiID: 10.1111/mpp.2017.18.issue-7 )
          : 937-948
          Affiliations
          [ 1 ] CNRS Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR2594 Castanet‐Tolosan 31326 France
          [ 2 ] INRA, Laboratoire des Interactions Plantes Microorganismes (LIPM), UMR441 Castanet‐Tolosan 31326 France
          [ 3 ] Laboratoire de Recherche en Sciences Végétales Université de Toulouse, CNRS, UPS; BP 42617 Auzeville Castanet‐Tolosan 31326 France
          [ 4 ] AgroParisTech Institut Jean‐Pierre Bourgin, Unité Mixte de Recherche 1318, Saclay Plant Science Versailles 78000 France
          [ 5 ] Laboratory of Phytopathology Plant Sciences Group, Wageningen University Droevendaalsesteeg 1 Wageningen PB 6708 the Netherlands
          Author notes
          [*] [* ] Correspondence: Email: canut@ 123456lrsv.ups-tlse.fr
          [†]

          These authors made equal contributions to this work.

          Article
          PMC6638305 PMC6638305 6638305 MPP12457
          10.1111/mpp.12457
          6638305
          27399963
          85881a6c-3848-4907-a77f-eeb645d86198
          © 2016 BSPP AND JOHN WILEY & SONS LTD
          History
          : 11 February 2016
          : 15 June 2016
          : 22 June 2016
          Page count
          Figures: 6, Tables: 0, Pages: 12, Words: 8466
          Categories
          Original Article
          Original Articles
          Custom metadata
          2.0
          mpp12457
          September 2017
          Converter:WILEY_ML3GV2_TO_NLMPMC version:5.6.4 mode:remove_FC converted:10.06.2019

          lectin, Pseudomonas ,jasmonic acid,Arabidopsis,plant–pathogen interactions,receptor‐like kinases

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