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      Comparison of fructification efficiency between reciprocal crosses in Coffea canephora Translated title: Comparación de la eficiencia de fructificación entre cruces recíprocos en Coffea canephora

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          Abstract

          Abstract This study aimed to assess and compare the behavior of superior genotypes of Coffea canephora in terms of fruiting capacity using directed hybridizations in a full diallel design with reciprocals. Hybridizations were performed using 12 possible combinations between genotypes 02, 03, 23 and 83 from the Incaper breeding program. High estimates of the genotypic coefficient of determination in terms of the total number of pinheads per cross (TNP) and fruit set (FS) indicated variability of genetic nature. FS averaged 52,27 %, ranging from 31,32 % to 82,09 %, with the higher rates relating to clone 23 as the male parent. Differences were found between reciprocal crosses in the combinations with genotype 23, which emerged as the donor plant with the best fruiting rate, but poor receiving ability. Clone 83 prevailed as the best receiving plant and the least effective donor. In the comparison of these two genotypes, antagonism was observed in their behavior when they were used as pollen-donor plants (male parents) and pollen-receiver plants (female parents), highlighting their possible maternal effect. Crosses involving clones 02 and 03 showed no differences when used reciprocally. The estimates of genotypic correlations were negative for the four associations involving the FS characteristic, being of greater magnitude with TNP.

          Translated abstract

          Resumen Este trabajo tuvo como objetivo evaluar y comparar la eficiencia en la producción de frutos entre cruces recíprocos en Coffea canephora con el uso de hibridaciones controladas en un dialelo completo con diseño recíproco. Las hibridaciones se realizaron involucrando los genotipos 02, 03, 23 y 83 del programa de mejoramiento Incaper. Las estimaciones altas del coeficiente de determinación genotípica para el número total de frutos iniciados por cruzamiento (TNP) y la proporción de frutos (FS) indicaron una variabilidad de naturaleza genética. La FS promedio fue de 52,27 %, con un rango de 31,32 % a 82,09 %, siendo mayor con el clon 23 como progenitor masculino. Se encontraron diferencias entre cruces recíprocos en combinaciones con el genotipo 23, que se destacó como la planta donante con la mejor tasa en la producción de frutos, pero con habilidad receptora deficiente. El clon 83 se destacó como la mejor planta receptora y la peor donante. En la comparación de estos dos genotipos (23 y 83) se observó antagonismo en su comportamiento al ser utilizados como plantas donadoras de polen (progenitores masculinos) y plantas receptoras de polen (progenitores femeninos), evidenciando un posible efecto maternal. Los cruces entre los clones 02 y 03 no mostraron diferencias cuando se usaron recíprocamente. Las estimaciones de las correlaciones genotípicas fueron negativas para las asociaciones que involucraron la característica FS, siendo de mayor magnitud con TNP.

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          Genes Software - extended and integrated with the R, Matlab and Selegen

          ABSTRACT. The Genes Software is useful for analyzing and processing phenotypic and molecular data using different biometric models. In the current version we dispose routines to integrate it with three other softwares: the R, Matlab and Selegen. This version allows in plant and animal breeding complementary analyzes in several breeding research fields as genomic selection, prediction of genetic values, use of neural networks and Fuzzy logic. The Genes is important to estimate parameters for understanding biological phenomena necessary to make decisions and predict the success and viability of strategic selection. The original programme can be downloaded in Portuguese, English or Spanish with the specific literature from (http://www.livraria.ufv.br/) and the user guide from (http://www.ufv.br/dbg/genes/genes.htm and http://www.ufv.br/dbg/biodata.htm). The user has also support in the address www.facebook.com/ GenesNews. The Genes is also integrated in the application softwares MS Word, MS Excel and Paint to efficiently import data and export results as numbers and figures.
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            Incompatibility in angiosperms

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              Inheritance and genetic mapping of self-incompatibility in Coffea canephora Pierre.

              Cross-compatibility behaviour of doubled haploid (DH) and hybrid genotypes of Coffea camphora was established using both phenotypic bioassay and in situ seed-set examination. The availability of DHs provided the opportunity of working with genetically homogenous pollen and female parents. The aniline blue fluorescence (ABF) method was applied to detect callose accumulation in pollen and pistil. Clear cross-compatibility/incompatibility situations were observed and confirmed by in situ seed-set analysis. Cross-compatibility analysis of hybrid combinations involving different DHs corroborated the crossing behaviour observed at the DH level. Expression of the self-incompatibility system did not appear to be affected by the low vigour of the DH. The crossing-behaviour distribution observed within DHs derived from clone IF200 confirmed that self-incompatibility in C. canephora is a gametophytic self-incompatibility system controlled by a single locus (S-locus). Reduced seed-set developments following incompatible crosses may indicate the occurrence of pseudo-incompatibility. Molecular marker linkage analysis showed that the S-locus is associated with an RFLP marker on linkage group 9. The availability of a linked DNA marker should facilitate the genetic analysis of self-incompatibility in relation to coffee breeding programmes.
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                Author and article information

                Journal
                acag
                Acta Agronómica
                Acta Agron.
                Universidad Nacional de Colombia (Palmira, Valle, Colombia )
                0120-2812
                June 2023
                : 72
                : 2
                : 161-167
                Affiliations
                [1] ES orgnameInstituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural-Incaper Brazil volpi@ 123456incaper.es.gov.br
                [2] Brasília Distrito Federal orgnameEmbrapa Café Brazil maria.ferrao@ 123456embrapa.br
                Article
                S0120-28122023000200161 S0120-2812(23)07200200161
                10.15446/acag.v72n2.97817
                568e9dc1-c477-4e82-a90f-200b04044610

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 17 August 2021
                : 09 October 2023
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 22, Pages: 7
                Product

                SciELO Colombia

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                number of pinheads,hybrid combinations,Espírito Santo,genetic parameters,conilon coffee,frutos en formación,parámetros genéticos,combinaciones híbridas,café conilon

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