CD45-assisted PanLeucogating for accurate, cost-effective dual-platform CD4+ T-cell enumeration.

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Abstract

North American and European guidelines for dual-platform (DP) flow cytometry recommend absolute CD4 T-cell counts to be calculated from two parameters: the absolute lymphocyte counts obtained on a hematology analyzer and the percentages of CD4+ cells among lymphocytes (CD4%/lympho) obtained by flow cytometry. Nevertheless, the identification of lymphocytes is error-prone: a poor match between these common denominators in the two systems is the main source of inaccuracy. In contrast, total leucocyte counts (white cell counts [WCC]) and CD4% among the gated CD45+ leucocytes (CD4%/leuco) can be determined with greater accuracy.

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Journal

Journal ID (iso-abbrev): Cytometry

Title: Cytometry

Publisher: Wiley

ISSN: 0196-4763

ISSN (Print): 0196-4763

Publication date (Electronic): Apr 15 2002

Volume: 50

Issue: 2

Affiliations

[1 ] Department of Molecular Medicine and Hematology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, PO Box 2551 Houghton, 2041, South Africa. glencross@mighty.co.za

Article

DOI: 10.1002/cyto.10068

PubMed ID: 12116348

SO-VID: 43c45286-625c-4c58-af29-801da6d4bec4

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