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      Identification and characterization of multipotential stem cells in immortalized normal ovarian surface epithelial cells : The pluripotency of immortalized normal ovarian surface epithelial cells

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          Abstract

          The ovarian surface epithelium (OSE) is a single layer of squamous-to-cuboidal epithelial cells that experience repetitive ovulatory rupture and subsequent repair. However, the characteristics of human immortalized ovarian surface epithelial cells (IOSE80) remain elusive. This study aims to determine whether IOSE80 cells have the characteristics of stem cell proliferation and multilineage differentiation and their application in regenerative medicine. IOSE80 cells are sequenced by high-throughput transcriptome analysis, and 5 sets of public data are used to compare the differences between IOSE80 cells and bone marrow mesenchymal stem cells, pluripotent stem cells, and oocytes in transcriptome profiling. The IOSE80 cells present a cobblestone-like monolayer and express the epithelial cell marker KRT18; the stem cell markers IFITM3, ALDH1A1, and VIM; lowly express stem cell marker LGR5 and germ cell markers DDX4 and DAZL. In addition, the GO terms “regulation of stem cell proliferation”, “epithelial cell proliferation”, etc., are significantly enriched ( P<0.05). IOSE80 cells have the potential to act as mesenchymal stem cells to differentiate into adipocytes with lipid droplets, osteoblasts, and chondroblasts in vitro. IOSE80 cells express pluripotent stem cell markers, including OCT4, SSEA4, TRA-1-60, and TRA-1-81, and they can be induced into three germ layers in vitro. IOSE80 cells also form oocyte-like cells in vitro and in vivo. In addition, IOSE80 cells exhibit robust proliferation, migration, and ovarian repair functions after in vivo transplantation. This study demonstrates that IOSE80 cells have the characteristics of pluripotent/multipotent stem cells, indicating their important role in tissue engineering and regenerative medicine.

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          Most cited references40

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          Mesenchymal and haematopoietic stem cells form a unique bone marrow niche.

          The cellular constituents forming the haematopoietic stem cell (HSC) niche in the bone marrow are unclear, with studies implicating osteoblasts, endothelial and perivascular cells. Here we demonstrate that mesenchymal stem cells (MSCs), identified using nestin expression, constitute an essential HSC niche component. Nestin(+) MSCs contain all the bone-marrow colony-forming-unit fibroblastic activity and can be propagated as non-adherent 'mesenspheres' that can self-renew and expand in serial transplantations. Nestin(+) MSCs are spatially associated with HSCs and adrenergic nerve fibres, and highly express HSC maintenance genes. These genes, and others triggering osteoblastic differentiation, are selectively downregulated during enforced HSC mobilization or beta3 adrenoreceptor activation. Whereas parathormone administration doubles the number of bone marrow nestin(+) cells and favours their osteoblastic differentiation, in vivo nestin(+) cell depletion rapidly reduces HSC content in the bone marrow. Purified HSCs home near nestin(+) MSCs in the bone marrow of lethally irradiated mice, whereas in vivo nestin(+) cell depletion significantly reduces bone marrow homing of haematopoietic progenitors. These results uncover an unprecedented partnership between two distinct somatic stem-cell types and are indicative of a unique niche in the bone marrow made of heterotypic stem-cell pairs.
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            Derivation of oocytes from mouse embryonic stem cells.

            Continuation of mammalian species requires the formation and development of the sexually dimorphic germ cells. Cultured embryonic stem cells are generally considered pluripotent rather than totipotent because of the failure to detect germline cells under differentiating conditions. Here we show that mouse embryonic stem cells in culture can develop into oogonia that enter meiosis, recruit adjacent cells to form follicle-like structures, and later develop into blastocysts. Oogenesis in culture should contribute to various areas, including nuclear transfer and manipulation of the germ line, and advance studies on fertility treatment and germ and somatic cell interaction and differentiation.
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              Ovarian surface epithelium: biology, endocrinology, and pathology.

              The epithelial ovarian carcinomas, which make up more than 85% of human ovarian cancer, arise in the ovarian surface epithelium (OSE). The etiology and early events in the progression of these carcinomas are among the least understood of all major human malignancies because there are no appropriate animal models, and because methods to culture OSE have become available only recently. The objective of this article is to review the cellular and molecular mechanisms that underlie the control of normal and neoplastic OSE cell growth, differentiation, and expression of indicators of neoplastic progression. We begin with a brief discussion of the development of OSE, from embryonic to the adult. The pathological and genetic changes of OSE during neoplastic progression are next summarized. The histological characteristics of OSE cells in culture are also described. Finally, the potential involvement of hormones, growth factors, and cytokines is discussed in terms of their contribution to our understanding of the physiology of normal OSE and ovarian cancer development.
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                Author and article information

                Journal
                Acta Biochim Biophys Sin (Shanghai)
                Acta Biochim Biophys Sin (Shanghai)
                ABBS
                Acta Biochimica et Biophysica Sinica
                Oxford University Press
                1672-9145
                1745-7270
                19 January 2024
                25 February 2024
                19 January 2024
                : 56
                : 2
                : 239-254
                Affiliations
                [1 ] orgnameThe International Peace Maternity and Child Health Hospital orgnameSchool of Medicine orgnameShanghai Jiao Tong University Shanghai 200030 China
                [2 ] orgnameShanghai Key Laboratory of Embryo Original Diseases Shanghai 200030 China
                Author notes
                Correspondences address. Tel: +86-21-64070434; laidongmei@hotmail.com
                Article
                10.3724/abbs.2023253
                10984850
                38243680
                41168b73-9081-43a2-a5d3-be059fb7c940
                © The Author(s) 2021.

                0

                This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/).

                History
                : 22 July 2023
                : 21 September 2023
                Funding
                Funded by: the grants from the National Natural Science Foundation of China
                Award ID: Nos.
                Award ID: 81971334
                Award ID: 82271664
                Funded by: the Research Projects of Shanghai Municipal Council for Science and Technology
                Award ID: No.
                Award ID: 20JC1412100
                Funded by: Shanghai Key Laboratory of Embryo Original Diseases
                Award ID: No.
                Award ID: Shelab2022ZD01
                Funded by: Shanghai Municipal Health Committee
                Award ID: No.202240345
                This work was supported by the grants from the National Natural Science Foundation of China (Nos. 81971334 and 82271664), the Research Projects of Shanghai Municipal Council for Science and Technology (No. 20JC1412100), Shanghai Key Laboratory of Embryo Original Diseases (No. Shelab2022ZD01) and Shanghai Municipal Health Committee (No.202240345).
                Categories
                Research Article
                Custom metadata
                L Hou, H Hong, W Cao, L Wei, L Weng, S Yuan, C Xiao, et al. Identification and characterization of multipotential stem cells in immortalized normal ovarian surface epithelial cells. Acta Biochim Biophys Sin, 2024, Vol.: fpage–lpage, https://doi.org/10.3724/abbs.2023253
                2024/1/16 17:21:28
                L Hou
                L Hou, H Hong, W Cao, L Wei, L Weng, S Yuan, C Xiao, et al.
                Identification and characterization of multipotential stem cells in immortalized normal ovarian surface epithelial cells

                ovarian surface epithelial cell,transcriptome,multipotential stem cell,oocyte,repair function

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