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      A Mouse with a Monoclonal Primary lmmunoglobulin Repertoire not Further Diversified by V-Gene Replacement

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          Abstract

          We have generated a monoclonal B-cell mouse by introducing homozygous, nonfunctional RAG-2 alleles and a λ1 light-chain transgene into the quasi-monoclonal (QM) mouse, which contains a “knocked-in” V HDJ H rearrangement. Thus, this mouse, which we call MonoB, is devoid of T cells and contains preformed heavy- and light-chain genes encoding immunoglobulin with an anti-NP specificity. The MonoB mouse allows us to examine immunoglobulin diversity in the absence of processes mediated by V(D)J recombination and T cells. Here we report that not only is the MonoB's primary immunoglobulin repertoire monoclonal, but also that its secondary repertoire is not further diversified by V-gene replacement or gene conversion. Among 99 heavy-chain and 41 λ light-chain genes from peripheral B cells of the MonoB mouse, there were no V-gene replacements. When compared to the QM mouse, which has RAG activity, and for which V-gene replacement is the major diversifying mechanism, these data suggest that V-gene replacement is mediated by V(D)J recombination and not by other recombination systems.

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          Author and article information

          Contributors
          gillian.wu@utoronto.ca
          Journal
          Dev Immunol
          Developmental Immunology
          Hindawi Publishing Corporation
          1044-6672
          1999
          : 7
          : 1
          : 43-50
          Affiliations
          [1 ] Department of Microbiology and Immunology University of California San Francisco CA 94143 USA
          [2 ] Department of Immunology Ontario Cancer Institute University of Toronto Toronto Ontario M5G 2M9 Canada
          [3 ] Ontario Cancer Institute Room 8-113 610 University Avenue Toronto Ontario M5G 2M9 Canada
          Article
          S1740252299245143
          10.1155/1999/24514
          2276038
          10636478
          2c1a9813-a5f2-4630-9725-e2e18b3111b9
          Copyright © 1999 Hindawi Publishing Corporation.

          This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

          History
          : 9 December 1998
          : 1 December 1998
          Categories
          Research Article

          Developmental biology
          monob mouse,qm mouse,rag,transgenic,knockin,flow cytometry
          Developmental biology
          monob mouse, qm mouse, rag, transgenic, knockin, flow cytometry

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