Differential expression patterns of OCC1-related, extracellular matrix proteins in the lateral geniculate nucleus of macaque monkeys

Abstract

The extracellular matrix (ECM) plays important roles in the development and plasticity of the central nervous system, and it has been shown that it regulates reorganization of the neuronal network. We have found that expression of OCC1, testican-1, testican-2, testican-3, SPARC and SC1 mRNAs, which encode members of the OCC1-related family of ECM proteins, exhibits distinct activity-dependent expression patterns in the adult macaque visual cortex. This finding suggests that OCC1-related proteins play crucial roles in the visual processing pathway. In the present study, we examined mRNA expression patterns of OCC1-related genes in the dorsal lateral geniculate nucleus (dLGN) of macaques. The mRNAs of testican-1 and testican-2 were strongly expressed in both excitatory projection neurons and GABAergic interneurons in the dLGN. Expression of testican-3 mRNA, which is predominantly observed in GABAergic interneurons in the cortex, was restricted to excitatory projection neurons in the dLGN. SPARC mRNA was strongly, and exclusively, expressed in glial cells in the dLGN. Interestingly, neuronal SC1 mRNA expression was abundantly observed in intercalated, koniocellular layers of the dLGN, while it was preferentially observed in blob regions of the primary visual area that receives color coding K-pathway projection from dLGN koniocellular layers, suggesting a pathway preference of expression. Finally, monocular inactivation experiments demonstrated that expression of testican-1, testican-2 and testican-3 mRNAs in the dLGN is dependent on sensory activity. Given their differential expression patterns and activity dependence, products of OCC1-related genes may modulate visual processing and plasticity at the level of the dLGN and the visual cortex.