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      Ovule ontogeny of Tabebuia pulcherrima Sandwith (Bignoniaceae): megasporogenesis and integument development Translated title: Ontogenia do óvulo de Tabebuia pulcherrima Sandwith (Bignoniaceae): megasporogênese e desenvolvimento do tegumento

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          Abstract

          The ovule of Tabebuia pulcherrima is anatropous, unitegmic and tenuinucellate. The nucellus shows a trizonate structural organization. The integument is initiated by periclinal divisions in the dermal layer (zone I), around the base of the archesporium. Subsequently, cells derived from the subdermal layer (zone II) start to push the dermal cells, so that they shift toward the micropylar region. The archesporial cell differentiates directly into the megaspore mother cell, undergoes meiosis, and originates a linear tetrad of megaspores. The mature embryo sac mother cell is elongated, possess a conspicuous central nucleus, and a characteristic bipolar vacuome with fibrous-granulated content. The inner cell layers of the integument differentiate into an amyloplast-rich endothelium. Patterns of callose deposition in the tetrad and selection of the functional megaspore, as well as the taxonomic value of some characters are discussed.

          Translated abstract

          Os óvulos de Tabebuia pulcherrima são anátropos, unitégmicos e tenuinucelados. Os nucelos apresentam estrutura trizonada. O tegumento é iniciado por divisões periclinais na camada dérmica (zona I), ao redor da base do arquespório. Em seguida, células derivadas da camada subdérmica (zona II) tomam parte na constituição do tegumento, empurrando as células de origem dérmica para a região micropilar. A célula arquespórica diferencia-se diretamente em megasporócito, sofre meiose e origina uma tétrade linear de megásporos. A célula-mãe do megásporo madura é alongada, com núcleo central e vacuoma bipolar apresentando conteúdo fibro-granular. As camadas celulares mais internas do tegumento diferenciam-se em endotélio rico em amiloplastos. Padrões de deposição de calose na tétrade e da seleção do megásporo funcional, bem como o valor taxonômico de alguns caracteres são discutidos.

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          Plant microtechnique

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            Comparative Embryology of Angiosperms

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              Callose in cell walls during megasporogenesis in angiosperms.

              Callose was detected by fluorescence microscopy in megasporogenesis in all investigated species with mono- and bisporic embryo-sac development. Callose occurs first in the meiotic prophase in the chalazal part of the megasporocyte wall and by the first meiotic metaphase the whole cell is enveloped in a callose-containing wall. Later, there is a marked decrease of callose fluorescence, usually at the chalazal end of the megasporocyte. In Oenothera, where the micropylar megaspore is active, decrease of fluorescence takes place at the micropylar pole of the megasporocyte. Callose appears centrifugally in the cell plates forming eventually the walls dividing the megaspores. It disappears from the walls of the megaspores during degeneration and differentiation.
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                Author and article information

                Journal
                rbb
                Brazilian Journal of Botany
                Braz. J. Bot.
                Sociedade Botânica de São Paulo (São Paulo, SP, Brazil )
                0100-8404
                1806-9959
                March 2002
                : 25
                : 1
                : 103-115
                Affiliations
                [01] Campinas SP orgnameUniversidade Estadual de Campinas orgdiv1Departamento de Botânica Brazil
                [02] Porto Alegre RS orgnameUniversidade Federal do Rio Grande do Sul orgdiv1Departamento de Botânica orgdiv2Laboratório de Anatomia Vegetal Brazil jorge.mariath@ 123456ufrgs.br
                Article
                S0100-84042002000100013 S0100-8404(02)02500113
                10.1590/S0100-84042002000100013
                0fad60c2-64e8-405a-adeb-30f8166985c3

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 16 January 2002
                : 24 January 2001
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 39, Pages: 13
                Product

                SciELO Brazil


                Bignoniaceae,megasporogenesis,ovule ontogeny,Tabebuia,Integument

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